IBP ... Bienvenue sur le site de l'IBP

Partenaires

IBP
CNRS
IFR 87
Université Paris-Sud
SPS

Rechercher

Sur ce site

Sur le Web du CNRS

Accueil du site > Equipes de Recherche > Génome et Développement > Chromatine et développement des plantes

Chromatine et Développement des Plantes/Chromatin and Plant Development

Chef d’équipe/Group leader : Dao-Xiu ZHOU

2 juillet 2012

Composition :

  • Dao-Xiu Zhou (Professeur)
  • Martin Kreis (Professeur)
  • Yves Deveaux (Maître de conférence)
  • Natalia Conde e Silva (Maître de conférence)
  • Yuan Shen (Doctorante/PhD student, Chinese Scholar Council)
  • Laure Audonnet (Doctorante/PhD student)
  • Caroline Servet (Postdoc, ANR)

THEMES DE RECHERCHE :

Le thème de recherche est focalisé sur l’étude de la régulation épigénétique de l’expression des gènes, du fonctionnement du génome et du développement chez Arabidopsis et le riz. Ces dernières années, nous avons étudié la fonction de plusieurs acétyltransférases (HAT), déacétylases (HDAC) et déméthylases (KDM) d’histones (e.g. Bertrand et al., 2003, JBC ; 2005, JBC ; Huang et al., 2007, Plant Physiol. ; Sun and Zhou, 2008, PNAS ; Qin et al., 2010, Mol Plant ; Kim et al., 2013, BBRC ; Chen et al., 2013, PloS Genet). Un mécanisme moléculaire d’activation à court terme (par la lumière) médiée par l’acétylation/déacétylation des histones a été proposé (Benhamed et al., 2006, Plant Cell ; Kim et al., 2009, Cell Res. ; Servet et al., 2010 ; Mol Plant). Les travaux sur les HDAC ont mis en avant l’important rôle de la déacétylation des histones dans les interactions épigénétiques entre les génomes "parents" pour le contrôle "non-additif" de l’expression des gènes chez des hybrides de riz (Li et al., 2011, PloS ONE). Nous avons étudié le rôle de la méthylation des histones et du variant d’histone H2A-Z dans l’activation précoce de l’expression de gènes (Hu et al., 2011, PloS ONE). Nos données montrent une persistance des modifications post-traductionnelles H3K4me3 et H3K27me3 après l’activation de l’expression de ces gènes. Ces résultats semblent indiquer que la méthylation des histones serait un marqueur de l’état de l’activité des gènes et non celui de l’activation/répression de l’expression des gènes. Récemment nous avons montré que la protéine CHD3 peut reconnaître et réguler l’état de méthylation de H3K4 et H3K27 sur les gènes peu exprimés chez le riz, révélant un mécanisme de lecture des combinaisons des modifications post-traductionnelles des histones par des protéines chromatiniennes (Hu et al., 2012, PNAS).

RESEARCH ACTIVITY :

The research activity of this laboratory is focused on the study of genetic and epigenetic control of gene expression, genome function and plant development in Arabidopsis and rice. During the last years, the group has studied the regulatory mechanisms of several histone acetyltransferases (HAT), deacetylases (HDAC) and histone demethylases in Arabidopsis and rice (e.g. Bertrand et al., 2003, JBC ; 2005, JBC ; Huang et al., 2007, Plant Physiol. ; Sun and Zhou, 2008, PNAS ; Qin et al., 2010, Mol Plant ; Kim et al., 2013, BBRC ; Chen et al., 2013, PloS Genet). A model for molecular mechanism of histone acetylation/deacetylation-mediated activation of short-term (e.g. light) regulated genes has been proposed (Benhamed et al., 2006, Plant Cell ; Kim et al., 2009, Cell Res. ; Servet et al., 2010 ; Mol Plant). Work on HDAC has revealed an important role of histone deacetylation in epigenetic interaction between parental genomes to control non-additive gene expression in hybrid rice (Li et al., 2011, PloS ONE).

We have evaluated the role of histone methylations and histone variant H2A.Z in rapid gene activation (Hu et al., 2011, PloS ONE). Our data show that changes of histone H3K4me3 and H3K27me3 levels lagged behind gene activation, suggesting that these methylation marks may play a role to label gene activity rather than to activate/repress gene transcription. Recently we have demonstrated that a CHD3 protein could recognize and regulate H3K4 and H3K27 methylation over under-expressed genes in the rice genome, revealing a reading mechanism of a combination of histone modification marks by chromatin proteins (Hu et al., 2012, PNAS).

Our ongoing work is orientated to study the acqisition, recongition and resetting mechanisms of epigenetic marks involved in cellular memory of gene expression related to plant development and stress-responses.

Keywords : Chromatin, Histone modifications, epigenetics, Plant development.

SUPPORTS : Our research programs have been supported by GENOPLANTE Programs (2001-2006) and more recently by the French Agence Nationale de Recherche (ANR) program "Blanc" : POLYCOMBARA (2008-2011), ANR blanc "CERES" (2011-2014). ANR blanc "NERDPATH" (2013-2015)

CONTACT : email : dao-xiu.zhou@u-psud.fr Tel : 33 (0)1 6915 3413

Representative Publications

DX. Zhou, Y. Hu, Y. Zhao (2013) Chapter "Epigenomics of rice" in Gentics and Epigenetics of Rice edited by Dr. Q. Zhang and Dr. Rod Wing , Springer.

X. Zhong, H. Zhang, Y. Zhao, Q. Sun, Y. Hu, P. Hai, & D-X Zhou (2013) The NAD+-dependent histone deacetylase OsSRT1 targets preferentially to stress and metabolism-related genes and transposable elements. Plos ONE (sous presse)

X. Chen, DX. Zhou (2013) Rice epigenomics and epigenetics : challenges and opportunities. Curr Opin Plant Sci. http://dx.doi.org/10.1016/j.pbi.201...

Q. Chen, X. Chen, Q. Wang, F Zhang, Z Lou, Q Zhang, DX Zhou (2013) Structural basis of a plant histone H3 Lysine 4 demethylase required for cell division in rice. PLOS Genet. 2013 Jan ;9(1):e1003239. doi : 10.1371/journal.pgen.1003239.

W Kim, D Latrasse, C Servet & DX Zhou (2013) Arabidopsis histone deacetylase HDA9 regulates flowering time through repression of AGL19. BBRC 432(2):394-8. doi : 10.1016/j.bbrc.2012.11.102.

Y. Hu , D. Liu, X. Zhong, C. Zhang, Q. Zhang, DX Zhou (2012) A CHD3 protein recognizes and regulates methylated histone H3 lysines 4 and 27 over a subset of targets in the rice genome. Proc. Natl. Acad. Sci. USA 109(15):5773-5778

Y. Hu, Y. Shen, N. Conde e Silva, DX. Zhou (2011) The role of histone methylation and H2A.Z occupancy during rapid activation of ethylene responsive genes. Plos One 2011 ; 6(11):e28224.

C. Li, L. Huang, C. Xu, Y. Zhao, DX. Zhou (2011) Altered levels of histone deacetylase OsHDT1 affect differential gene expression patterns in hybrid rice. PloS One 2011 ; 6(7):e21789.

X. Chen, Y. Hu, DX. Zhou (2011) Epigenetic gene regulation by plant Jumonji group of histone demethylase. Biochim Biophys Acta. 1809(8) : 421-426.

F. Qin, Q. Sun, L. Huang, X. Chen, DX. Zhou (2010) Rice SUVH histone methyltransferases genes display specific functions in chromatin modification and retrotransposon repression Mol. Plant 3(4):773-782.

C. Servet, N. Conde e Silva, DX. Zhou (2010) Histone acetyltransferase AtGCN5/HAG1 is a versatile regulator of developmental and inducible gene expression in Arabidopsis. Mol. Plant 3(4) : 670-677.

DX. Zhou and Y. Hu (2010) Control of gene expression and plant growth in rice by histone modification regulation. Rice 3, 103-111.

Y. Zhao Y. Hu, M. Dai, L. Huang and DX. Zhou (2009) The Wuschel-related homeobox gene Os-WOX11 is required to activate shoot-borne crown root development in rice. Plant Cell 21(3):736-748.

Y. Hu, F. Qin, L. Huang, Q. Sun, C. Li, Y. Zhao, DX. Zhou (2009) Rice histone deacetylase genes display different expression pattern and developmental function. Biochem Biophys Res Commun. 388(2) : 266-271.

W. Kim, M. Benhamed, C. Servet, D. Latrasse, W. Zhang, M. Delarue, DX. Zhou (2009) Histone acetyltransferase GCN5 interferes with the miRNA pathway in Arabidopsis. Cell Res. 19(7) : 899-909.

C. Servet, M. Benhamed, D. Latrasse, W. Kim, M. Delarue, DX. Zhou (2008) Characterization of a phosphatase 2C protein as an interacting partner of the histone acetyltransferase GCN5 in Arabidopsis. Biochim Biophys Acta 2008 1779(6-7) : 376-382.

Q. Sun & DX. Zhou (2008) The rice jmjC domain-containing gene JMJ706 encodes a histone H3 lysine 9 (H3K9) demethylase required for floral organ development. Proc. Natl. Acad. Sci. USA 105(36):13679-84.

DX. Zhou (2008) Regulatory mechanism of histone epigenetic modification in plants. Epigenetics 4(1):15-18.

M. Benhamed, ML. Martin-Magniette, L. Taconnat, F. Bitton, C. Servet, ..... JP. Renou, DX. Zhou, P. Hilson (2008) Genome-scale Arabidopsis promoter array identifies targets of the histone acetyltransferase GCN5. Plant J. 56(3):493-504.

L. Huang, Q. Sun, F. Qin, C. Li, Y. Zhao & DX. Zhou (2007) Down-regulation of a Silent Information Regulator2-related histone deacetylase gene SRT1 induces DNA fragmentation and cell death in rice. Plant Physiol. 144, 1508-1519.

M. Dai, Y. Hu, Y. Zhao, H. Liu & DX. Zhou (2007) A rice WUSCHEL-LIKE HOMEOBOX (WOX) gene represses a YABBY gene expression required for shoot development. Plant Physiol. 144, 380-390.

M. Dai, Y. Zhao, Q. Ma, Y. Hu, P. Heden, Q. Zhang & DX. Zhou (2007) The Rice YAB1 transcription factor gene is involved in the feedback regulation of gibberellin metabolism. Plant Physiol. 144, 121-133.

M. Benhamed, C. Bertrand, C. Servet & DX. Zhou (2006) Arabidopsis GCN5, HD1 and TAF1/HAF2 Interact to Regulate Histone Acetylation Required for Light-Responsive Gene Expression. Plant Cell 18, 2893-2903.

C. Bertrand, M. Benhamed, Y.-F. Li, M. Ayadi, G. Lemonier, J.P. Renou, M. Delarue, DX. Zhou (2005) Arabidopsis HAF2 gene encoding TATA-binding protein (TBP)-associated factor TAF1, is required to integrate light signals to regulate gene expression and growth. J. Biol. Chem. 280 : 1465-1473.

S. Guyomarc’h, C. Bertrand, M. Delarue, DX. Zhou (2005) Regulation of meristem activity by chromatin remodeling, Trends Plant Sci, 10, 332-338.

C. Bertrand, C. Bergounioux, S. Domenichini, M. Delarue & DX. Zhou (2003) - Arabidopsis histone acetyltransferase AtGCN5 regulates the floral meristem activity through the WUSCHEL/AGAMOUS Pathway. J. Biol. Chem. 278 : 28246-28251.